Anagen Technologies, Inc. offers a variety of Mutagenesis services. Mutagenesis is performed by generating mutations in cloned segments of DNA. This is done by either chemical and enzymatic based methods. At Ana-Gen, we can produce mutations at an extremely high efficiency (approaching 100% in some cases). Essentially any type of mutation can be generated.
Oligonucleotide-directed mutagenesis |
Oligonucleotide-directed mutagenesis uses a mutagenic oligonucleotide primer to alter the DNA template sequence in a defined way. Oligonucleotides can also be used to create a large number of mutations within specific regions of DNA. However, sometimes it is more efficient to do GeneSynthesis if many different mutations are desired in the same fragment of DNA. By using specific chemicals, it is possible to generate many random mutations in fragments as large as 3 kb. Linker-scanning mutagenesis is used to introduce clusters of point mutations throughout a particular sequence. The polymerase chain reaction (PCR) method can be used to introduce restriction sites or point mutations into specific DNA sequences. |
The prices for mutagenesis service depends upon the requirements of each customer.
|
PCR based point mutations can be as low as $800 per mutation (>10 reactions per order). The standard price for this service is $950-$2,500 per mutation. This includes design, synthesis, purification of mutagenic oligonucleotide primers, PCR amplification, transformation, plasmid isolation and sequence verification. The final product is provided either as a stab, purified plasmid, glycerol stock or on plates. |
Note: The introduction of a large number of mutations in a single DNA is more efficiently performed by GeneSynthesis, and can cost $3,000 to $10,000 per gene. We have successfully synthesized genes >1kb. Please call (4040 223-5090 or email us for more information.
|